[Poster] High-throughput lipidomic analysis of arachidonic acid metabolites in biological fluids by liquid chromatography/mass spectrometry
Arachidonic acid metabolites are biologically active lipid molecules that regulate many functions and play critical roles in a variety of physiological and pathophysiological processes. Some of the eicosanoids are used as a potential biomarkers related to inflammation, immune reactions and side-effects of drugs. Eicosanoids are derived from arachidonic acid and then from other metabolites by enzymatic and non-enzymatic reactions and have very similar structure and physical properties. Pattern of intermediates and final eicosanoids production is determined in a stimulus and cell-specific fashion. Fast and simultaneous qualitative and quantitative profiling of eicosanoids (lipidomics) in biological samples is possible because of high sensitivity and selectivity of liquid chromatography/mass spectrometry methods and simplicity of sample preparation.