Small Molecule Bioanalysis

Small Molecule Bioanalysis at CMIC USA

Small Molecule Bioanalysis at CMIC

At CMIC, we are experts in small molecule bioanalysis, offering comprehensive services essential for drug development, pre-clinical, and clinical research. Our expertise provides valuable insights into the pharmacokinetics, metabolism, and toxicity of small molecule compounds in biological systems. These small molecules include pharmaceutical drugs, metabolites, endogenous compounds, and other biomolecules with low molecular weights. The process is typically supported by chromatographic assays.

Supporting Clients with:

  • Sample Preparation
  • Chromatographic Analytical Techniques
  • Quantification and Calibration Curves
  • Quality Control Samples
  • Data Analysis and Reporting

Learn more about how we can support your small molecule projects. Contact us to get started.

At CMIC’s Chicago based bioanalysis laboratory, we offer cutting-edge small molecule bioanalysis services tailored to your specific needs. Our experienced team and state-of-the-art facilities ensure precise and dependable results, helping you make informed decisions and accelerate your research.

Sample preparation is essential to remove proteins, lipids, salts, and other interfering substances, isolating and concentrating the molecules of interest while minimizing matrix effects. Techniques we employ include:

  • Protein Precipitation: Removing proteins by adding a precipitating agent, followed by centrifugation.
  • Solid-Phase Extraction (SPE): Separating analytes using a solid adsorbent material.
  • Liquid-Liquid Extraction (LLE): Partitioning analytes between two immiscible liquids.
  • Filtration Techniques: Removing particulate matter and impurities.

Chromatographic separation techniques coupled with various analytical detectors are commonly used in small molecule bioanalysis. CMIC Group excels in utilizing these techniques, which include:

Separation Techniques:

  • Liquid Chromatography (LC): Separating components in a mixture through a column packed with a solid adsorbent.
  • High-Performance Liquid Chromatography (HPLC): Using high pressure to push the solvent through the column for improved separation efficiency.
  • Ultra-High-Performance Liquid Chromatography (UHPLC): Operating at even higher pressures for faster and more efficient separations.

Detection Techniques:

  • Mass Spectrometry (MS): Identifying and quantifying compounds based on their mass-to-charge ratio.
  • Fluorescence Detection (FD): Utilizing the fluorescent properties of compounds for detection and quantification.
  • Ultraviolet (UV) Detection: Using UV light to detect compounds based on their absorbance properties.

Quantification in small molecule bioanalysis involves comparing signals from the analytes in the sample to known standards or reference materials. CMIC Group excels at establishing calibration curves to determine the relationship between analyte concentration and detector response, which are essential for:

  • Establishing Linear Ranges: Determining the concentration range over which the detector response is linear.
  • Quantifying Analytes: Accurate determination of analyte concentrations in samples.

Quality control (QC) samples with known concentrations of analytes are analyzed alongside study samples to assess the accuracy and precision of the analytical method. At CMIC Group, QC samples are used to:

  • Verify Method Performance: Ensure reliable and reproducible results.
  • Monitor Data Integrity: Detect any deviations or inconsistencies in the analysis.

Validated systems and software collect and process data from sample analysis. A Laboratory Information Management System (LIMS) manages, regresses, and reports these data. CMIC excels in:

  • Calibration Curve Fitting: Describing the relationship between analyte concentration and detector response.
  • Peak Integration: Measuring the area under the chromatographic peak for each analyte.
  • Quantification: Determining analyte concentrations based on the calibration curve.
  • Quality Control Checks: Verifying accuracy and precision through QC samples.

Partner with CMIC Group

Have inquiries about our services or are you interested in outsourcing your operations to us?